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課程名稱 |
胚胎顯微操作與應用
Embryo Micromanipulation |
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開課學期 |
111-2 |
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授課對象 |
生物資源暨農學院 生物科技研究所 |
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授課教師 |
宋麗英 |
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課號 |
Biot7022 |
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課程識別碼 |
642EM0230 |
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班次 |
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學分 |
3.0 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期三2,3,4(9:10~12:10) |
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上課地點 |
生技所415 |
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備註 |
本課程以英語授課。修習過生殖生理學與進階動物生物技術相關基礎課程者。
限碩士班以上
總人數上限：6人 |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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為確保您我的權利,請尊重智慧財產權及不得非法影印
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課程概述 |
Understand the early embryo development and modern reproductive biotechnologies |
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課程目標 |
This course will focus on mouse animal model to provide students a state-of-the- art of the technologies in embryo development and pluripotent stem cell (i.e. embryonic stem cell) research and its applications. The course will provide valuable opportunities for students to participate various practical training and knowledge in oocyte and embryo handling, in vitro fertilization, cryopreservation of egg and embryo, micromanipulation techniques (i.e. intracytoplasmic sperm injection and somatic cell nuclear transfer, embryo biopsy, blastocyst injection…etc), derivation and in vitro/in vivo characterizations of pluripotent stem cells. |
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課程要求 |
A background in developmental biology or reproductive biology is required. |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
1. Developmental Biology, eighth edition, Scott F. Gibert, Sinauer Associates, Inc., USA
2. Reproduction in Farm Animals, seventh edition, E.S.E.Hafez and B. Hafez, Lippincott Williams and Wilkins
3.Pathways to pregnancy and parturition, second edition, P.L. Senger, Current Conceptions, Inc. USA
4. PubMed: http://www.ncbi.nlm.nih.gov/pubmed/ |
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評量方式
(僅供參考) |
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週次 |
日期 |
單元主題 |
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Week 1 |
2/22 |
Course introduction and discussion |
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Week 2 |
3/01 |
Collection of mouse oocytes |
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Week 3 |
3/08 |
Invited speaker: Nuwa Doctor, Dr. Chi Chun Liao |
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Week 4 |
3/15 |
In vitro fertilization and embryo culture |
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Week 5 |
3/22 |
Invited speaker: Nuwa Embryologist, Ms. Ying Jie Chen |
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Week 6 |
3/29 |
Vitrification of mouse oocytes and embryo freezing |
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Week 7 |
4/05 |
Spring break |
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Week 8 |
4/12 |
Oocyte and embryo thawing |
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Week 9 |
4/19 |
Derivation of mouse embryonic stem cells |
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Week 10 |
4/26 |
Pick up outgrowth and subculture of ESCs |
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Week 11 |
5/03 |
Tetraploid embryo complementation/2-cell fusion |
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Week 12 |
5/10 |
Blastocyst injection/embryo transfer |
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Week 13 |
5/17 |
Mouse intracytoplasmic sperm injection (ICSI) |
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Week 14 |
5/24 |
Pronuclear microinjection |
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Week 15 |
5/31 |
Somatic Cell Nuclear Transfer (SCNT) |
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Week 16 |
6/07 |
Student presentation and discussion |
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Week 17 |
6/14 |
Field trip (sometime in May, Tuesday 5:00pm): Nuwa Fertility Center 宜蘊生殖醫學中心 中央胚胎實驗室 |
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